Design de primers



Primer3 is a complete rewrite of the original PRIMER programs(Primer 0.5), written by Steve Lincoln, Mark Daly, and EricsLander. See DIFFERENCES FROM EARLIER VERSIONS for a discussionsof how Primer3 differs from its predecessors, Primer 0.5 andsPrimer v2.ssPrimer3 picks primers for PCR reactions, considering as criteria:sso oligonucleotide melting temperature, size, GC content,s and primer-dimer possibilities,sso PCR product size,sso positional constraints within the source sequence, andsso miscellaneous other constraints.s

Remarque s
Run Unix # primer3_core [-format_output] [-2x_compat] [-strict_tags]Run Web #

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