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Summary of the sequencing project

Sequencing summary

The sequencing project was launched in Mars 2000 and ended in Mars 2003. A shotgun library of 8,500 clones of an average insert size of 1,3 kb was constructed and sequenced in three steps, forward, reverse and custom. Each step was followed by sequence assembly with the phred, phrap and consed suite softwares from the University of Washington and subsequent autofinish analysis. Gap closure was carried out in four steps: vectorette PCR, genomic comparison, multiplex PCR and sequencing, physical mapping. Gap closure was carried out only with PCR reactions. At least three PCR products were sequenced on both strands for each gap. Some assembly statistics are accessible.